Bisazole, which is prepared by homocoupling of imicazole derivatives bearing olefinic alkylene substituent on the nitrogen atom, gives E-selective macrocyclic olefin by Ring-closing metathesis with Ruthenium (Grubbs') catalyst. Formation of the macrocyclic structure is confirmed by X-ray analysis. This compound results in molecular asymmetry (axially chiral) and separation of the enantiomers can be separated by HPLC with chiral column.